學生姓名:
蘇家靖
指導教授:
陳詠宗
學期:
114下
摘 要:
Tea is recognized for its anti-aging potential, attributed to bioactive compounds such as polyphenols and flavonoids. However, the compositional differences among tea cultivars and their underlying molecular mechanisms remain to be fully elucidated. This study utilized LC/UPLC-MS/MS-based metabolomic profiling to characterize the phenolic and flavonoid landscapes across diverse tea types. The biological efficacy was evaluated using Caenorhabditis elegans as an in vivo model to assess lifespan, oxidative stress, and gene expression patterns. Results demonstrated that the aqueous extract of Xianhu tea (XHT) possessed a significantly higher polyphenol content, featuring 23 characteristic phenolic markers. In C. elegans, XHT treatment effectively suppressed the accumulation of ROS, MDA, and lipofuscin while bolstering SOD and CAT activities. These interventions extended lifespan by 23.50% and 21.07%, effects that were associated with the regulation of 13 aging-related genes. Comparative analysis across six major tea categories identified dark tea (DT) as the most potent anti24 aging candidate. Beyond modulating classical pathways such as IIS, SKN-1/HSF-1, and AMPK/SIRT1/FOXO, DT treatment upregulated skn-1 and ftn-1, reduced intracellular Fe²⁺ levels, and attenuated lipid peroxidation-related damage while maintaining mitochondrial structural and functional integrity. These studies have shown that the longevity-promoting effects of tea extracts are driven by the synergistic coordination of antioxidant defense, mitochondrial protection, and ferroptosis inhibition. The divergent anti-aging efficacies observed among tea varieties are closely associated with the chemical composition and concentrations of their active phenolic and flavonoid components.
蘇家靖.pdf