學生姓名:
林桂英
指導教授:
吳奕賢
學期:
114下
摘 要:
Coffee quality and safety are significantly influenced by microbial activity during fermentation as well as contamination byand by contamination with toxic compounds such as ochratoxin A (OTA). OTA is a mycotoxin mainly produced by Aspergillus and Penicillium species and is associated with serious health risks, including nephrotoxicity and carcinogenicity. Its high stability under acidic conditions, resistance to thermal processing, and long biological half-life make it difficult to eliminate once formed, thereby posing a significant challenge to the coffee industry. The first study evaluates the application of wild yeasts isolated from fermentation environments in inhibiting the germination of ochratoxin-producing fungi during coffee pulp fermentation. The findings demonstrate that metabolites produced by Saccharomyces cerevisiae exhibit the strongest and most consistent inhibitory effects on fungal spore germination, suggesting that antifungal activity is mediated by metabolic byproducts rather than the presence of live microorganisms. The second study presents an advanced plasmonic immunosensor based on surface plasmon resonance (SPR) technology for the ultra-sensitive detection of OTA in coffee samples. The biosensor demonstrates an exceptionally low detection limit in the parts-per-trillion range, high selectivity against structurally similar compounds and common coffee constituents, and strong performance in real sample analysis without significant matrix interference. Its simple design, portability, and ability to operate without complex instrumentation make it highly suitable for on-site applications. This report aims to highlight innovative strategies for both preventing contamination and improving the detection of toxins, contributing to enhanceddetecting toxins, thereby enhancing coffee safety and quality control.
林桂英.pdf