學生姓名:
江立荷
指導教授:
林泓廷
學期:
114下
摘 要:
Antibiotic resistance is a major global public health issue, and overexpression of the AcrAB-TolC efflux pump is a key mechanism underlying multidrug resistance in Escherichia coli. In this study, Sargassum ilicifolium extracts obtained using different polarity solvents and microwave-assisted extraction (MAE) were evaluated for their potential as efflux pump inhibitors (EPIs). The highest extraction yield (11%) was achieved using 50% ethanol with 15 min microwave extraction. The extracts themselves did not exhibit significant direct antibacterial activity, which is consistent with the low-toxicity characteristics expected for EPIs. Checkerboard assays identified six optimal conditions that significantly reduced the MIC values of erythromycin, chloramphenicol, and ciprofloxacin, with modulation factors reaching up to 8-fold. Ethidium bromide (EtBr) fluorescence accumulation assays indicated limited direct inhibition of the AcrB efflux pump; however, slight fluorescence increases at low concentrations suggested that the synergistic effects may involve weak efflux pump inhibition, altered membrane permeability, or enhanced antibiotic penetration. Overall, Sargassum ilicifolium extracts demonstrate potential as antibiotic potentiators and warrant further investigation into their active compounds and mechanisms of action.
江立荷.pdf