學生姓名:
邱品禎
指導教授:
林泓廷
學期:
115上
摘 要:
Vibrio parahaemolyticus is a leading foodborne pathogen causing bacterial gastroenteritis, with multidrug-resistant (MDR) strains posing serious public health threats and economic losses in marine aquaculture, particularly white shrimp (Penaeus vannamei) production. Understanding the genomic diversity, transmission vectors, and virulence traits of MDR strains is crucial for effective risk assessment and control. In this study, whole-genome sequencing (WGS) and phylogenomic analyses were conducted on foodborne and aquaculture-derived MDR V. parahaemolyticus isolates. We analyzed their genetic diversity, sequence types (STs), virulome, and resistome. Mobile genetic elements (MGEs), particularly conjugative plasmids driving the dissemination of antimicrobial resistance genes (ARGs), were functionally characterized. Phylogenomic profiling revealed high genetic diversity among the isolates, identifying distinct clades and novel STs. Genomic screening identified critical virulence factors (including T3SS-1, T3SS-2, and hemolysin genes $tdh$/$trh$) across diverse lineages. Broad-spectrum resistomes targeting beta lactams, tetracyclines, and aminoglycosides were frequently localized on conjugative plasmids, facilitating highfrequency horizontal gene transfer across strains. Our findings highlight the pivotal role of plasmid-mediated horizontal transfer in fueling the spread of MDR V. parahaemolyticus in food systems and shrimp aquaculture. Integrated WGS and phylogenomic surveillance are urgently needed to monitor these genomic traits and prevent the further dissemination of resistance in aquatic food supplies.
邱品禎.pdf