Effect of lactoferrin on osteoarthritis and application

學生姓名: 張恆偉
指導教授: 龔瑞林
學　　號: 0013A034
學期: 104上
摘　　要: Osteoarthritis (OA) is a debilitating disease characterized by degradation of articular cartilage, osteophyte formation and synovitis. Although its etiology remains understood, numerous studies have indicated the progression of OA, including enhanced expression of collagenases, aggrecanases and activation of pro-inflammatory cytokine-mediated pathways. Lactoferrin (Lf) is 78~80 kDa iron-binding glycoprotein having multifunctional properties ranging from anti-microbial, anti-oxidative to anti-carcinogenic and anti-inflammatory. The aim of this study may understand the effect of lactoferrin on osteoarthritis and application.
The first study was to investigate mRNA expression for matrix metalloproteases (MMPs) and inhibitor of metalloproteinase-1 (TIMP-1) of normal human articular chondrocytes stimulated with interlukin-1β (IL-1β, 10 ng/mL) and tumor necrosis factor-α (TNF-α, 10 ng/mL), and the study found that IL-1β and TNF-α up-regulated MMP-1, MMP-3 and down-regulated TIMP-1 at the same time. Secondly, the study discussed the function of bovine lactoferricin (LfcinB) to human articular chondrocytes. Results showed LfcinB significantly restored proteoglycan loss caused by IL-1β in articular cartilage and antagonized production of MMPs in the inflammatory signaling pathways. The last study evaluated the effect of iron saturated-bovine lactoferrin encapsulated chitosan nanocarriers (AEC-CP-Fe-bLF-NC). Results suggested that AEC-CP-Fe-bLF-NC not only decreased cell toxicity and apoptotic activity induced by IL-1β but also promoted nuclear proliferative and glycosami-noglycan release of chondrocytes.
In conclusion, IL-1β and TNF-α might result in inflammation of OA. AEC-CP-Fe-bLF-NC reduced these pro-inflammatory cytokine-mediated pathways and modulated absorption rate in the intestine. Hence, it shall be utilized in the treatment of chronic inflammatory diseases such as OA in the future.