學生姓名:
韋季萱
指導教授:
洪良邦
學 號:
00239203
學期:
104上
摘 要:
Sudan dyes are classified as the category 3 carcinogens by the International
Agency for Research on Cancer. These colorants have been proven through laboratory experiments which cause tumors in the liver or bladder of rats, mice, and rabbits; they are also considered as a possible genotoxic carcinogen and mutagen source to humans. Hence their use as food additives is prohibited worldwide at any level. Due to their economic benefits, they have been illegally added into certain food products such as chili and red pepper, however, causing risks to public health. Developing reliable methods to detect the presence of these compounds are necessary.Studies regarding the detection of Sudan dyes were carried out, namely, high performance liquid chromatography (HPLC) with acidified water, acetonitrile and methanol, ionic liquid/anionic surfactant aqueous two-phase (ATPS) extraction coupled with HPLC, solid phase extraction (SPE) coupled with HPLC-DAD. For these methods, their limits of determination (LOD) were 0.23-0.34 mg/L, 3.68-5.45 μg/kg, and 4.1-5.8 μg/kg; their limits of quantification (LOQ) were 1.19-1.7 mg/L,12.3-18.2 μg/kg, and 13.2-19.1 μg/kg; recovery rates were 62.3-77.6%, 82.3-112.1 %, and 93.2-103 %; and the extraction time were 16 mins, 19 mins, and 26 mins respectively. The three methods proposed by various authors have been proven suitable in terms of "detecting Sudan dyes".
專題討論 2016.01.12 韋季萱 摘要.pdf