學生姓名:
曾揚捷
指導教授:
江孟燦
學期:
107下
摘 要:
Sulfated polysaccharide was isolated from Monostroma oxyspermum through hot water extraction, anion-exchange and gel permeation column chromatography. The sulfated polysaccharide contained 92% ofcarbohydrate, 0% of protein, 7.8% of uronic acid, 22% of ash and 33% of moisture respectively. The elemen-tal composition was analyzed using CHNS/O analyzer. The molecular weight of sulfated polysaccharidedetermined through PAGE was found to be as 55 kDa. Monosaccharides analysis revealed that sulfatedpolysaccharide was composed of rhamnose, fructose, galactose, xylose, and glucose. The structural fea-tures of sulfated polysaccharide were analyzed by NMR spectroscopy. Further the sulfated polysaccharideshowed total antioxidant and DPPH free radical scavenging activity were as 66.29% at 250 g/ml and66.83% at 160 g/ml respectively. The sulfated polysaccharide also showed ABTS scavenging ability andreducing power were as 83.88% at 125 g/ml and 15.81% at 400 g/ml respectively. The anticoagulantactivity was determined for human plasma with respect to Activated Partial Thromboplastin Time (APTT)and Prothrombin Time (PT) was 20.09 IU and 1.79 IU at 25 g/ml respectively. These results indicated thatthe sulfated polysaccharide from M. oxyspermum had potent antioxidant and anticoagulant activities.
2014-Structural characterization and bioactivities of sulfated polysaccharide from Monostroma oxyspermum.pdf