學生姓名:
曾揚捷
指導教授:
江孟燦
摘 要:
The goal for this work was to characterize basic biological properties of a novel Arthrospira platensis-based
aqueous cyanophyta extract (ACE), enriched in the known anti-inflammatory cyclooxygenase-2 (COX-2) inhibitor phyco-
cyanin (PC), but also containing a high level of non-PC bioactive compounds. Antioxidant properties were tested in parallel in
the Folin–Ciocalteu assay (chemical antioxidant capacity) and in the cellular antioxidant protection (CAP-e) bioassay, where
both the PC and the non-PC fractions contributed to the antioxidant capacity and CAP of ACE. In contrast to the COX-2
inhibition seen in the presence of PC, the inhibition of enzymatic activity of the inflammatory mediator Lipoxygenase was
associated specifically with the non-PC fraction of ACE. Inhibition of formation of reactive oxygen species (ROS) was
evaluated using polymorphonuclear cells from healthy human donors. The inhibition of ROS formation was seen for both the
PC and non-PC fractions, with ACE showing the most robust effect. The effects of PC, non-PC, and ACE on clotting and clot
lysing was tested using a modified Euglobulin fibrinolytic assay in vitro. In the presence of PC, non-PC, and ACE, the time for
clot formation and lysis was not affected; however, the clots were significantly more robust. This effect was statistically
significant ( p < .05) at doses between 125–500 lg/mL, and returned to baseline at lower doses. Both PC and the non-PC
fraction contributed to the antioxidant properties and anti-inflammatory effects, without a negative impact on blood clotting in
vitro. This suggests a potential benefit for the consumable ACE extract in assisting the reduction of inflammatory conditions.
2015-Antioxidant_and_Anti-Inflammatory_Properties_of_an_Aqueous_ Cyanophyta_Extract_Derived_from_Arthrospira_Platensis_Contributi on_to_Bioactivities_by_the_Non-Phycocyanin_Aqueous_Fraction.pdf