學生姓名:
姜珮文
指導教授:
吳彰哲
學 號:
10832018
學期:
108下
摘 要:
Mulberry (Morus alba L.) has been used is Chinese medicine for preventing and curing type 2 diabetes (Chan et al., 2016). It shows that 1-deoxynojirimycin, one of the polysaccharide which extracts from mulberry, can be one of the α-glucosidase (Liu et al., 2016). Skeletal muscle accounts for approximately 70–80% of insulin-stimulated glucose uptake and it’s important in maintaining glucose homeostasis (Carnagarin et al., 2015). There are two mainly pathways for glucose transport in skeletal muscle: phosphatidylinositol-3 kinase (PI3 kinase)/protein kinase B (Akt) and AMP-activated protein kinase (AMPK). Insulin stimulates glucose uptake by increasing the translocation of glucose transporter 4 (GLUT4) from an intracellular pool to the plasma membrane through the activation of the PI3K/Akt pathway (Saltiel et al., 2001). The two studies are respectively used the mulberry lead extract (MLE) and mulberry anthocyanin extract (MAE), to investigate the pathway that the samples stimulate the glucose uptake increasing by insulin in vivo and in vitro, measuring the Akt, AMPK, GLUT4 and others indication in serum, including fasting blood glucose (FBG), insulin, adiponectin et al. First, MLE is able to increasing the Akt, AMPK, GLUT4 in myotube cells and in db/db mice, which is also decreasing the FBG, insulin, adiponectin in vivo. Second, MAE not only can increase the Akt, AMPK, glycogen synthase kinase (GSK) and glycogen synthase (GYS), but also decreasing the PPAPγ coactivator (PGC-1α) and glucose-6-phosphate (G6P), the two enzymes to overcome the gluconeogenesis. However, not only MLE but also MAE are able to activates the Akt and its downstream targets in insulin-sensitive tissues, which are associated with glucose homeostasis, and they may serve as a strategy to improving the insulin resistance and type 2 diabetes mellitus.
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