學生姓名:
江和光
指導教授:
黃意真
學期:
109下
摘 要:
Inflammation is a response of the immune system to harmful stimuli, which acts to remove injurious stimuli and initiate the healing process. The aim of this study was to develop a macrophage-targeted nanoparticles composed of hyaluronan/fucoidan complexes with polyethylene glycol-gelatin to encapsulate and deliver epigallocatechin-3-gallate (EGCG). Nanoparticles (NPs) can successfully bond to macrophages and deliver more EGCG than an EGCG solution treatment. The targeting abilities of CD44 binding were increased as the hyaluronan concentration increased and decreased by adding a competitor CD44 antibody. Fucoidan treatment significantly reduced macrophage migration in a dose-responsive manner. Three different fucoidan/chitosan NPs were developed for the topical delivery of methotrexate (MTX) towards the treatment of skin-related inflammatory diseases. MTX loaded in 3F/1C and 5F/1C NPs did not affect cells viability and presented lower cytotoxicity. MTX-loaded F/C NPs lead to a significant reduction of pro-inflammatory cytokines. Skin permeation studies showed that NPs permeated the pig ear skin barrier reaching after 6 h are better than free MTX. Studies showed that fucoidan complex nanoparticles can effectively inhibit inflammation.
摘要_江和光 (1).odt