學生姓名:
劉佳姍
指導教授:
黃崇雄
學 期:
112上
摘 要:
Allergic rhinitis represents a global health problem. Allergic rhinitis affects 10% to 20% of the population worldwide, and the incidence of allergic rhinitis has been steadily on the rise. Allergic rhinitis is a common disorder associated with Type I hypersensitivity reactions, mediated by immunoglobulin E (IgE). It activates T helper 2 (Th2) cells and leads to the secretion of a significant amount of cytokines, such as interleukin (IL)-4, IL-5, and IL-13. At present, the animal models of allergic rhinitis are variously established. Establishing satisfactory animal models is critical for the development of health food and effective treatments for allergic rhinitis. Therefore, this study aims to investigate the impact of mouse strains, allergen sensitization methods, and concentrations on the allergic rhinitis model. According to the results of these papers, BALB/c mice showed higher Th2 response and serum immunoglobulin levels than C57BL/6 mice. The study found 25 µg to be a more appropriate dose for Dermatophagoides farinae 1 (Der f1) sensitization. On the other hand, within a certain concentration range, ovalbumin (OVA) concentration positively related to the severity of symptoms, IgE antibody level, and Th2 bias. Meanwhile, serum antibodies (IgE and IgG1) and cytokines (IL-4, IL-5 in spleen and IL-4 in nasal lavage fluid, NALF) were significantly higher in the classical intraperitoneal (IP) injection group than in the nasal drip groups. In summary, BALB/c mice are more suitable for use in allergic rhinitis models compared to C57BL/6 mice. Additionally, it has been demonstrated that immunotolerance develops after exposure to allergens at a certain dosage. Furthermore, it has been confirmed that differences in mouse strains and allergen sensitization methods correlate with the severity of tissue inflammation and cytokine expression levels.
11232005_劉佳姍_專題討論摘要.pdf