學生姓名:
邵詩淳
指導教授:
王上達
學 期:
112上
摘 要:
Brown algae is a valuable source of bioactive compounds, particularly phlorotannins, which are phenolic compounds containing phloroglucinol units, and exhibited significant bioactivity, making them a promising area for research. This study aims to investigate the optimal conditions for extracting phlorotannins from seaweeds and further explore the potential health benefits of their extract. According to the results of these reports, the extraction of phlorotannins was evaluated with particular emphasis on the influential parameters, including
the solvent concentration (X1), solvent-solid ratio (X2), extraction temperature (X3) and extraction time, using a single-factor design followed by a Box-Behnken design. The maximum total phlorotannins content, determined using the 2,4-dimethoxybenzaldehyde (DMBA)
method, corresponded to 2.92 ± 0.05 mg of phloroglucinol equivalents/g dry seaweed (mg PGE/g DS), and was achieved for extracts carried out with 67% acetone concentration, a solvent-to-solid ratio of 70 mL/g, and a temperature of 25°C. On the other hand, the optimal
solvents for extraction was studied, and the extracts were subsequently evaluated for their potential bioactivity. The results showed that the best antioxidant capacity, inhibition of digestive enzymes α-amylase and pancreatic lipase, and anti-inflammatory potential through
the hindering of albumin denaturation were performed by ethyl acetate fraction. In summary, this research aims to identify the optimal conditions for extracting phlorotannins from algae and demonstrates the potential of phlorotannins in physiological activities, including α-amylase and pancreatic lipase inhibition and antioxidant properties. This provides a theoretical basis for the development of bioactive components from these two algae and further understanding of the exact structural features of phlorotannins.
專題討論20231025-邵詩淳-摘要.pdf