Methodology and microbial growth are discussed in this course. The methods are discussed including design of media, culture and preservation of microorganisms, evaluation of growth, cell extract, reaction system, and analysis of products. In microbial growth, growth dynamics, growth and its regulation, biochemical energetics, carbohydrated metabolism, electron transport, and biosynthesis are illustrated in detail. The course is mainly design for graduate students to undertake researches in physiology of microorganisms.