Inactivation effects of different antimicrobial substances against pathogens in fresh produce during washing and sanitation process
學生姓名:
陳字雲
指導教授:
蕭心怡
學期:
110上
摘 要:
The aim of this study was to review the current investigation of the efficacy of antimicrobial substances on controlling or inactivate pathogens in fresh produce during washing and sanitation process. The investigation was done by inoculating E. coli and Listeria spp., on fresh lettuce and Listeria spp., in apple. The water used for washing and sanitizing processes was treated by adding antimicrobial substances, including lactic acid/phosphoric acid-based antimicrobial (LPA), neutral electrolyzed oxidizing water (NEOW), sodium acid sulfate (SAS), and chlorine. The efficacy of these antimicrobials was evaluated using confocal laser scanning method (CLSM), widefield bioluminescence imaging, and microbial analysis test. The results of this review shows that (i) the use of NEOW shows greater reduction against E. coli and L. monocytogenes in lettuce only under low volume processing, (ii) increasing the volume of water will significantly increase the population of E. coli and L. monocytogenes in the fresh produce under the washing and sanitation process, (iii) the use of LPA shows greater reduction only against L. monocytogenes, (iv) The number of bacterial cells on the disks decreased as the high shear stress process, while the increase of exposure times will significantly increase the population of the initial pathogen. In this case, the contact time crucial than the shear stress in inactivation of the pathogen, (v) SAS at 1.0% demonstrated a better efficacy than 25 ppm chlorine, indicate that SAS could be the alternative antimicrobials to be
used after the chlorine ones. In conclusion, soaking fresh produce with NEOW and SAS demonstrated them as the greatest antimicrobials to inactivate E. coli and L. monocytogenes during the washing and sanitation process.
used after the chlorine ones. In conclusion, soaking fresh produce with NEOW and SAS demonstrated them as the greatest antimicrobials to inactivate E. coli and L. monocytogenes during the washing and sanitation process.
專題討論_20220105_陳字雲（摘要）.pdf