薑黃素 (Curcumin) 是來自薑黃的多酚化合物，具有多種生理活性如抗發炎、抗氧化及抗癌等；褐藻醣膠 (Fucoidan) 主要由 L-fucose 和硫酸酯基團組成，為具有多種生理活性的硫酸多醣。本實驗製備薑黃素-褐藻醣膠/幾丁聚醣奈米粒，以幾丁聚醣奈米粒共遞送薑黃素及褐藻醣膠，探討這些有效成分之特性及釋放，並應用於腸上皮屏障功能障礙之改善。當薑黃素、褐藻醣膠與幾丁聚醣重量比為 0.3:1:1.4 時，奈米粒粒徑約為 300 nm，Zeta 電位則為 +30 mV。薑黃素-褐藻醣膠/幾丁聚醣奈米粒具有 pH 值敏感性及良好的包覆率，於仿胃酸環境 (pH 1.2、pH 2.5) 中較穩定，粒徑範圍約在 300 nm；在仿腸道環境 (pH 7.4) 中奈米粒則有崩解的現象，粒徑增大至 873 nm 並釋放出薑黃素及褐藻醣膠。以穿透式電子顯微鏡觀察奈米粒形態，在 pH 1.2、pH 2.5及 pH 6.0 時奈米粒呈球型，pH 7.4 時則有明顯崩解現象。相較於薑黃素-褐藻醣膠奈米粒，薑黃素-褐藻醣膠/幾丁聚醣奈米粒在薑黃素濃度 ˂ 17.18 μM 具有良好的抗氧化能力；還具有較佳的生物相容性；亦能有效降低以脂多醣誘導 RAW264.7 細胞產生之 NO、IL-6 及 TNF-α 含量。細胞跨膜電阻及異硫氰酸螢光素-聚葡醣細胞間細通透性實驗結果顯示，薑黃素-褐藻醣膠/幾丁聚醣奈米粒可減少腸上皮細胞因脂多醣誘導發炎產生的破壞。綜合上述，薑黃素-褐藻醣膠/幾丁聚醣奈米粒具有抗氧化及抗發炎之功效，且能控制釋放薑黃素及褐藻醣膠，有效改善脂多醣誘導之腸屏障的破壞。

摘要 i
Abstract ii
目錄 iii
圖目錄 iv
表目錄 vi
第一章、前言 1
第二章、文獻回顧 2
2.1發炎腸道疾病 2
2.2腸上皮屏障 2
2.3腸道疾病與發炎反應 2
2.3-1腸上皮細胞凋亡 3
2.3-2腸上皮緊密連接的破壞 3
2.4薑黃素 4
2.4-1薑黃素結構 4
2.4-2薑黃素生理活性 5
2.3-3薑黃素的應用與缺點 6
2.5褐藻醣膠 6
2.5-1褐藻醣膠奈米粒 7
2.6口服傳輸系統 7
2.6-1幾丁聚醣奈米載體 7
第三章、實驗動機與目的 9
第四章、實驗架構 10
第五章、實驗流程 11
第六章、實驗材料與方法 12
6.1實驗材料 12
6.2實驗藥品 12
6.3實驗儀器 14
6.4實驗溶液配製 16
6.5實驗方法 17
第七章、結果與討論 27
第八章、結論 58
第九章、參考文獻 59

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