臺灣博碩士論文加值系統

本研究目的是將萃取自 Ulva lactuca 之石蓴多醣 (Ulvan, U) 製成薄膜，利用交聯的方式改善薄膜物化特性並分析其生物活性。石蓴多醣硫酸根含量為 33.3%，醣醛酸含量為 40.1%，經高效能液相層析儀分析分子量約為 2200 kDa，主要單醣為鼠李糖 (Rhamnose) 含量為 1024.4 μmol/g。石蓴多醣具有體外抗氧化活性，對小鼠纖維母細胞 (L929) 和人類角質細胞 (HaCaT) 無細胞毒性，美白活性分析能抑制酪胺酸酶酵素活性及抑制小鼠黑色素瘤細胞 (B16F10) 生長。以環氧化物 1,4-丁二醇二縮水甘油醚 (1,4-Butanediol diglycidyl ether, BDDE) 共價交聯石蓴多醣形成薄膜，兩者摩爾比為 1：25 時拉伸強度為 0.735 MPa；以幾丁聚醣 (Chitosan, C) 離子交聯石蓴多醣形成薄膜，當兩者重量比為 3：1 時，拉伸強度為 1.092 MPa，加入三聚磷酸鹽 (Tripolyphosphate, T) 和甘油 (Glycerol, G) 後，拉伸強度提高至 2.674 MPa，機械強度最佳。萃取石蓴中葉綠素 (Chlorophyll, Chl) 添加至薄膜中，可改變薄膜顏色作為天然之染劑。石蓴多醣複合薄膜相較於幾丁聚醣膜有較好的膨潤率、水氣透濕性和體外降解率，加入三聚磷酸鹽後能提高石蓴多醣釋放率達 41-65%，利用 Power Law 方程式進行釋放曲線的模擬，石蓴多醣複合薄膜在短時間內能利用擴散方式釋放石蓴多醣。石蓴多醣複合薄膜皆具有體外抗氧化活性，包括清除 DPPH 自由基和超氧陰離子自由基、亞鐵離子螯合能力以及還原力，細胞相容性實驗中，石蓴多醣複合薄膜對於 L929 和 HaCaT 無細胞毒性，在美白活性方面，石蓴多醣複合薄膜能抑制酪胺酸酶活性，抑制小鼠黑色素瘤細胞 (B16F10) 的生長，經流式細胞儀測量可能與誘導 B16F10 胞內活性氧物質生成有關，在抗氧化和美白活性中，薄膜表現出較游離之石蓴多醣更好的效果。綜合上述，石蓴多醣複合薄膜具有良好機械性質、膨潤及水氣透濕性、細胞相容性、抗氧化和美白的能力，極具應用潛力。

The aim of this study was to prepare ulvan (extracted from Ulva lactuca) films, to improve the physical and chemical properties of the films by cross-linking methods and analyze its biological activities. Ulvan has 33.3% sulfate groups and 40.1% uronic acid. Rhamnose (1024.4 μmol/g) is the main monosaccharide existed in ulvan. The molecular weight of ulvan was approximately 2200 kDa detected by high performance liquid chromatography. Ulvan exhibited antioxidant activity, biocompatibility, and inhibited tyrosinase enzyme activity and mouse melanoma cell (B16F10) growth. The ulvan complex film was successfully prepared using 1,4-Butanediol diglycidyl ether or chitosan as a cross-linker. The tensile strength of ulvan complex films increased to 2.674 MPa after crosslinked with chitosan and adding tripolyphosphate and glycerol. Furthermore, adding tripolyphosphate to the films increased ulvan release; adding chlorophyll, extracted from Ulva lactuca, to the film changed the color. Compared with chitosan films, ulvan complex films had better swelling, water vapour transmission rate and degradation rate. Tripolyphosphate can increase the release of ulvan by 41-65%, and the release curve can be fitted by Power Law equation. The ulvan complex films can release ulvan in a short time by diffusion method. The ulvan complex films had antioxidant activities, including DPPH radicals, superoxide radicals, ferrous ion scavenging activity, and reducing power and good biocompatibility with L929 and HaCaT cells. In terms of whitening efficacy, the films inhibited tyrosinase activity and the growth of B16F10, which may be attributed to the intracellular production of reactive oxygen substance in cells. Among the antioxidant activities and whitening efficacy, the films exhibited better effects than free ulvan. Briefly, the ulvan complex film has a great potential to be use as a new material.

目錄
摘要 I
Abstract II
目錄 i
圖目錄 ii
表目錄 iv
第一章、前言 1
第二章、文獻回顧 2
2.1 藻類 2
2.2 石蓴 2
2.3 石蓴多醣 3
2.4 交聯劑 5
2.5 葉綠素 8
2.6 皮膚介紹 9
2.7 皮膚老化 10
2.8 皮膚美白 12
第三章、實驗動機與目的 14
第四章、實驗架構 15
第五章、實驗流程 16
第六章、實驗材料與方法 17
6.1 實驗材料 17
6.2 實驗藥品 17
6.3 實驗儀器 19
6.4 實驗溶液配製 22
6.5 實驗方法 23
第七章、結果討論 36
7.1 石蓴之一般成分分析 36
7.2 石蓴多醣成分分析 36
7.3 石蓴多醣複合薄膜物化性質分析 42
7.4 細胞相容性 58
7.5 抗氧化能力 61
7.6 美白能力 65
第八章、結論 71
第九章、參考文獻 72
第十章、附錄 83

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