摘要
本研究選擇含細辛之中藥製劑辛夷散，建立其高效液相層析法，用於馬兜鈴酸之鑑別。辛夷散和細辛兩者以乙醇和水萃取物進行比較研究，經實驗結果顯示辛夷散和細辛使用C-18 之層析管，以乙腈(acetonitrile)/冰醋酸水溶液2％(glacial acetic acid)1:1(v/v)為移動相，其分離效果良好。為aristolochic acid I的檢測提供了一種靈敏、經濟、可行的科學方法。檢量線之相關係數均為0.999以上，而aristolochic acid I 在儀器之最低檢出量為0.05 mg，添加回收率則介於96.17％～98.65％之間。

Abstract
A high performance liquid chromatography (HPLC) for the determination of aristolochic acid in Chinese medicinal herbs has been studied. Xin Yi San was examined in detail and aristolochic acid contents in both ethanol and water extracts were also compared in this study. The samples (Xin Yi San and Asiasarum) were analyzed using the HPLC method on a C-18 column (Cosmosil 5 C 18-AR-II 4.6×250 mm) and were detected at 395nm with acetonitrile and 2% glacial acetic acid aqueous solution (1：1) (V/V), as mobile phase at a flow rate of 1.0 mL/min. This method was a sensitive, economic, scientific and feasible method for measuring aristolochic acid I. All the linear regression equation coefficients were greater than 0.999, detection limits was 0.05 mg. The recoveries of samples were 96.17%~98.65%.

目 錄
頁次
中文摘要　　　　　　　　　 　　　　　　 　 　Ⅰ
英文摘要　　　　　　　　 　　 　　 　 　 Ⅱ
目 錄　　　　　　　　 　　 　　 　 　 Ⅲ
表 目 錄　　　　　　　　 　　 　　 　 　 Ⅴ
圖 目 錄　　　　　　　　 　　 　　 　 　 Ⅵ
第一章、前言　　　 　　　 　 　　 　　 　 1
第二章、文獻整理　 　　　　 　　　　 2
一、細辛之介紹 2
二、含細辛之方劑 4
三、馬兜鈴酸之結構與馬兜鈴酸之功效 5
四、致癌代謝機制 8
五、食用馬兜鈴酸所引起之病變及動物試驗 10
六、我國對馬兜鈴科使用之相關規定 12
七、馬兜鈴酸萃取條件與分析方法 13
第三章、實驗架構（一）、設備與研究方法 18
一、實驗架構（二） 　 19
二、設備及材料 20
三、試藥及溶液配製 21
四、實驗步驟 22
五、ＨＰＬＣ 分析條件之建立 　 23
六、高效液相層析條件 24
七、同日間(Intra­­―­­­­day)及異日間(Inter―day)試驗 　　24
八、回收率試驗 25
九、偵測極限 25
第四章、結果與討論 27
第五章、結論 48
第六章、參考文獻 49
表目錄
Table 1辛夷散和細辛藥材添加馬兜鈴酸回收率 35
Table 2 線性回歸相關係數 36
Table 3 aristolochic acid標準品RSD值 38
Table 4精密度試驗　 39
Table 5穩定度試驗　 39
圖目錄
Fig 1馬兜鈴酸I和Ⅱ之基本結構 　 　 7
Fig 2馬兜鈴酸致癌代謝途徑 9
Fig 3馬兜鈴酸標準品HPLC圖譜 27
Fig 4馬兜鈴酸標準品HPLC圖譜 28
Fig 5馬兜鈴酸標準品HPLC圖譜 29
Fig 6馬兜鈴酸標準品HPLC圖譜 30
Fig 7馬兜鈴酸標準品HPLC圖譜 31
Fig 8馬兜鈴酸標準品HPLC圖譜 32
Fig 9 aristolochic acid標準品在水中飽和時上層液HPLC圖譜 34
Fig 10 aristolochic acid標準品檢量線 37
Fig 11 aristolochic acid標準品偵測極限圖譜 40
Fig 12細辛藥材乙醇萃取HPLC圖譜 41
Fig 13細辛藥材水萃取HPLC圖譜 42
Fig 14辛夷散品牌（A）乙醇萃取HPLC圖譜 43
Fig 15辛夷散品牌（B）乙醇萃取HPLC圖譜 44
Fig 16辛夷散品牌（A）水萃取HPLC圖譜 45
Fig 17辛夷散品牌（B）水萃取HPLC圖譜 46

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