臺灣博碩士論文加值系統

摘要 I
Abstract II
縮寫表 IV
目錄 V
表目錄 VII
圖目錄 VIII
第一章 前言 1
第二章 文獻回顧 2
第一節　頭頸癌 2
第二節　NME1 8
第三節　蜆肉水解物應用於頭頸癌治療 11
第三章 研究目的 14
第四章 研究方法 15
第一節　蜆肉水解物之製備 15
第二節 蜆肉水解物成分分析 15
第三節　細胞培養 16
第四節　錐蟲藍排除法 (Trypan blue exclusion assay) 17
第五節　分析細胞週期 (cell cycle analysis) 17
第六節　細胞蛋白質萃取 18
第七節　蛋白質定量 18
第八節　西方墨點法 (Western blotting) 19
第九節　免疫螢光染色法 20
第十節　Hexokinase (HK) 活性分析 21
第十一節　Phosphofructokianse (PFK) 活性測定 22
第十二節　Pyruvate kinase (PK) 活性測定 22
第十三節　細胞遷移試驗 23
第十四節 Matrix metalloproteinases (MMPs) 活性測定 23
第十五節 細胞內ROS含量測定 24
第十六節 統計分析 25
第十七節　cDNA微陣列分析及基因表現量統計 25
第五章　結果與討論 26
第一節、蜆肉水解物之成分 26
第二節、蜆肉水解物抑制SAS細胞增生 26
第三節、蜆肉水解物降低NME1低表現SAS細胞之cisplatin抗性 27
第五節、NME1調節SAS細胞之細胞週期及凋亡蛋白之基因表現 29
第六節、蜆肉水解物延緩NME1調節之SAS細胞遷移能力 30
第七節、蜆肉水解物輔助NME1抑制SAS細胞遷移能力之機轉 31
第八節、NME1調節SAS細胞葡萄糖轉運蛋白SLC2A3之基因表現及分佈 34
第六章、結論 39
第七章、未來展望 40
第八章、圖表 41
參考文獻 66
附錄 74

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