本研究主要探討不同褐藻醣膠產品可通用之相關檢測規格及指標成分含量。蒐集十一種市售之褐藻醣膠產品測量其醣醛酸、硫酸根及總糖含量，接著以體外抗氧化實驗(DPPH和ABTS自由基清除能力) 比較其抗氧化能力，並進一步以高效液相層析儀(High Performance Liquid Chromatography, HPLC) 分析褐藻醣膠之指標成分-岩藻糖含量。
結果顯示醣醛酸含量介於0.15 ± 0.00~19.44 ± 0.20 g/100 g，硫酸根含量方面介於0.21 ± 0.01~20.73 ± 0.46 g/100 g，最高檢測值皆為以日本沖繩褐藻作原料之樣品A；總糖含量則介於0.06 ± 0.01~681.91 ± 16.61 mg/g，以日本沖繩褐藻作原料之樣品J檢測值最低。體外抗氧化能力方面，DPPH 自由基清除能力及ABTS自由清除能力以樣品E最佳。而岩藻糖含量為樣品A檢測值最高，含量達40.21 ± 0.33 g/100 g。
本研究確立不同褐藻醣膠產品可通用之相關檢測規格以及透過調查得知市售褐藻醣膠產品標示之法規符合性。

In this study, we investigated the general testing specifications and indicative ingredient concentrations of different fucoidan products in Taiwan market. Eleven kinds of commercially available fucoidan products were collected to measure the content of uronic acid, sulfate and total sugar, then compared with in vitro antioxidative capacities (DPPH and ABTS free Radical scavenging ability), and further analyze the cconcentrations of the fucose, index component of fucoidan by high performance liquid chromatography.
The concentrations of uronic acid were between 0.15 ± 0.00~19.44 ± 0.20 g/100 g, the concentrations of sulfate were 0.21 ± 0.01~20.73 ± 0.46 g/100 g. Among them, the highest value was sample A which used Okinawa brown algae as raw material. The total sugar concentrations were between 0.06 ± 0.01~681.91 ± 16.61 mg/g, and the sample J showed the lowest level. In vitro antioxidative capacity tests, sample E was the best one for DPPH and ABTS free radical scavenging abilities. The fucose content was the highest 40.21 ± 0.33 g/100 g in sample A.
This study established the relevant test specifications for different fucoidan products and the regulatory compliance of the commercially available fucoidan products.

壹、 前言 ..............................................................................................................1
貳、 文獻回顧 ......................................................................................................2
一、 保健食品之介紹 ..................................................................................2
(一) 保健食品定義 ..................................................................................2
(二) 保健食品之發展 ..............................................................................2
(三) 藻類保健食品相關法規 ..................................................................2
二、 褐藻醣膠之介紹 ..................................................................................3
三、 褐藻醣膠之成分分析 ..........................................................................4
四、 抗氧化活性 ..........................................................................................4
(一) 自由基與活性氧 ..............................................................................4
(二) 活性氧與疾病之相關性..................................................................6
五、 褐藻醣膠之應用 ..................................................................................6
(一) 免疫活性 ..........................................................................................6 (二) 抗腫瘤及抗癌活性 ..........................................................................6
(三) 抗氧化能力 ......................................................................................7 (四) 抗血栓及抗凝血 ..............................................................................7 (五) 抗病毒 ..............................................................................................8
(六) 抗發炎 ..............................................................................................8
(七) 抗沙門氏菌 ......................................................................................9
(八) 抗幽門螺桿菌 ..................................................................................9
(九) 不同分子量褐藻醣膠之研究 ..........................................................9
參、 實驗架構 ....................................................................................................11
肆、 材料及方法 ................................................................................................12
II
一、實驗材料 ............................................................................................12
(一) 樣品 ................................................................................................12
(二) 抗氧化能力測試之試藥 ................................................................12
(三) 指標成分檢測之試藥 ....................................................................12
(四) 儀器與設備 ....................................................................................13
二、 實驗方法 ............................................................................................14
(一) 指標成分檢測方法 ........................................................................14
(二) 抗氧化活性分析 ............................................................................15
伍、 結果與討論 ................................................................................................17
一、 褐藻醣膠產品特性 ............................................................................17
二、 褐藻醣膠產品標示及法規符合性 ....................................................17
三、 醣醛酸含量 ........................................................................................17
四、 硫酸根含量 ........................................................................................17
五、 總糖含量 ............................................................................................18
六、 岩藻糖含量 ........................................................................................18
七、 DPPH自由基清除能力.....................................................................19
八、 ABTS自由基清除能力.....................................................................20
九、 褐藻醣膠產品與指標成分之比較 ....................................................20
陸、 結論 ............................................................................................................22
柒、 參考文獻 ....................................................................................................23
捌、 表 ................................................................................................................30
玖、 圖 ................................................................................................................34
壹拾、 附錄 ........................................................................................................48

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