本研究旨在開發一種具 pH 及溫度敏感的創傷敷材，全球對於傷口治療的需求依然不斷，薑黃素 (Curcumin) 作為一種特性優良的天然多酚類活性物質，已被廣泛應用傷口癒合。然而由於其具疏水性、易受光熱裂解導致穩定性差等缺點，本研究使用自玉米中萃取的天然聚合物玉米醇溶蛋白 (Zein) 作為兩親性化合物包覆薑黃素，以促進其水溶性，並以自石蓴 (Ulva lactuca) 中萃取的石蓴多醣 (Ulvan) 表面塗層增加穩定性，玉米醇溶蛋白與石蓴多醣於質量比 1 : 2下製備之奈米粒擁有最佳的包覆率、載藥率及儲存穩定性，傅立葉轉換紅外光譜 (FTIR) 及 X 光繞射分析 (XRD) 結果表明薑黃素成功包覆於玉米醇溶蛋白/石蓴多醣奈米粒當中，首次開發出了包覆薑黃素的玉米醇溶蛋白/石蓴多醣奈米粒，粒徑為228.30 ± 0.95 nm，Zeta 電位為 -29.90 ± 1.54 mV，且本研究發現製備之奈米粒具有 pH 敏感特性，在模擬傷口初期的中偏鹼性環境下(pH 7.4) 粒子崩解使藥物較快速釋放，而在中後期酸性環境下 (pH 5.0) 粒子呈球型從而緩慢釋放，可適用於不同的傷口階段，薑黃素-玉米醇溶蛋白/石蓴多醣奈米粒具抗發炎、抗氧化及促進細胞遷移活性。將奈米粒嵌入具溫度敏感特性之 Pluronic F127 / F68 / P123三組分 Pluronic 混合物中，以流變儀測定其凝膠溫度為 27.5℃，接近人體皮膚溫度，並具有合適的機械強度及降解率，其釋放具有良好細胞相容性、抗氧化及促進細胞遷移活性。綜上所述，本研究製備的奈米粒複合水凝膠具有應用於傷口修復之潛力。

This study aims to develop a novel pH-sensitive and thermo-sensitive wound dressings. The global demand for wound healing remains constant.Curcumin, ,as a natural polyphenolic active substance with excellent properties, has been widely used in wound repair. However, due to its hydrophobicity and poor stability, its effectiveness was limited.This study utilized Zein, a natural polymer extracted from corn, as a hydrophobic compound to encapsulate curcumin, enhancing its water solubility. Furthermore, a coating of ulvan, a polysaccharide extracted from Ulva lactuca, was applied to improve the stability of the curcumin-loaded zein nanoparticles.
Nanoparticles with a Zein-to-Ulvan mass ratio of 1:2 exhibited optimal encapsulation efficiency, loading content, and storage stability. Fourier-transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analysis confirmed the successful encapsulation of curcumin within the zein/ulvan nanoparticles. The developed zein/ulvan nanoparticles had an average particle size of 228.30 ± 0.95 nm and a zeta potential of -29.90 ± 1.54 mV. Moreover, the nanoparticles demonstrated pH-sensitive behavior, where the nanoparticles exhibited rapid drug release in a mildly alkaline environment simulating the early stages of wound healing (pH 7.4) and slow, sustained release in the acidic environment of the later stages (pH 5.0). The curcumin-loaded zein/ulvan nanoparticles demonstrated anti-inflammatory, antioxidant, and cell migration-promoting activities. To further enhance their application, the nanoparticles were embedded with thermo-sensitive Pluronic F127/F68/P123 hydrogel with a gelation temperature of 27.5°C. They display suitable mechanical strength, degradation rate, and cell compatibility. In conclusion, the prepared hydrogel of Curcumin-Zein/Ulvan nanoparticles holds great potential for wound repair applications.

摘要 I
Abstract II
Graphic Absract III
目錄 i
圖目錄 iii
表目錄 v
第一章、前言 1
第二章、文獻回顧 3
2.1 皮膚 3
2.2 傷口修復 4
2.3 傷口修復水凝膠 5
2.4 石蓴多醣 6
2.5 薑黃素 9
2.6 玉米醇溶蛋白奈米粒 10
第三章、實驗動機與目的 13
第四章、實驗架構 14
第五章、實驗材料與方法 15
5.1 實驗材料 15
5.2 實驗藥品 17
5.3 實驗儀器 19
5.4 實驗溶液配製 21
5.5 實驗方法 23
第六章、結果與討論 32
第七章、結論 68
第八章、未來工作 68
第九章、參考文獻 69
第十章、附錄 81

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