The proposes of this course were to introduce the structure, nomenclature, classification, biochemical properties and enzyme activity assay, the purification technique and methods, and the application. Therefore, this course included classification and nomenclature, binding energy and catalysis, biological electron transfer, steady state kinetics, enzyme metabolism, metal ions in enzymes, use of protein engineering to study enzyme mechanism, methods and technique of enzyme purification, heat stable enzymes, hydrolytic enzymes in animals, Immobilization of enzymes, and enzymes in food processing.