Study on the antioxidative and apoptosis-inducing activities of 2 peptides from clam
學生姓名:
陳佳琳
指導教授:
張君如
學期:
110上
摘 要:
Bivalve mollusks have high nutritional value related to protein content which possess anticancer and antioxidant properties. This report determined the effects of marine bivalve mollusks on oxidative damage and apoptosis. Xu et al. (2020) hydrolyzed Corbicula fluminea with trypsin, and then fractionated with ultrafiltration. The F5000 had the highest yield (68%), and relatively strong scavenging activity. Moreover, the component I, F5000 separated with a gel filtration column, showed the highest DPPH radical scavenging activity, while it increasing the activity of catalase, decreasing lipid peroxidation, inhibiting H2O2-induced apoptosis, maintaining normal cell cycle, and higher yield. Furthermore, the component I was purified and the peptide sequence was identified as KGPAPFYPL with molecular weight of 988.3 Da. In summary, purified peptide of C. fluminea had positive effects on oxidative damage in HepG2 cells. Sahayanathan and Chinnasamy (2021) using anion exchange chromatography to purify protein fraction from Donax variabilis. Protein fractions were dialyzed and lyophilized as PPF-V. The results showed that PPF-V significantly inhibited cell viability of non-small cell lung cancer (NSCLC) cells which IC50 value was 30 μg/ml in A549 cells, and 75 μg/ml in NCI-H23 cells. Subsequently, the results of MALDI-TOF/ MS revealed that PPF-V was highest similarity with FMRFamide like peptides. In addition, treatment with PPF-V induced ROS generation, mitochondrial membrane potential decreasing, cytochrome c releasing, and lead to apoptosis on
NSCLC cells. In summary, PPF-V induced apoptosis via mitochondrial-mediated pathway in NSCLC cells. Taken together, clam peptides have the potential as natural antioxidants and anticancer drugs.
NSCLC cells. In summary, PPF-V induced apoptosis via mitochondrial-mediated pathway in NSCLC cells. Taken together, clam peptides have the potential as natural antioxidants and anticancer drugs.
專題討論摘要20211013_陳佳琳.pdf