Immunomodulatory Effects of Polysaccharides from Auricularia auricular and Lentinula edodes on Raw 264.7 Macrophages
學生姓名:
劉承賢
指導教授:
陳建利
學期:
112下
摘 要:
Auricularia auricula and Lentinula edodes are edible fungi widely consumed. Research has indicated that their polysaccharide possess immunomodulatory activities; however, studies exploring the correlation between their molecular weight and immunomodulatory efficacy are
limited. Structural characteristics revealed that Auricularia auricula polysaccharide (AAP) was a homogeneous galactan comprising mannose, rhamnose, gluconic acid, glucose, galactose, arabinose, and fucose, and the average molecular weight is approximately 23.51 kDa. At
concentrations of 0.3-0.5 mg/ml, AAP does not produce toxicity in RAW 264.7 cells. After adding AAP, the increase in NO release from the RAW 264.7 cells does not lead to excessive inflammatory responses. The addition of AAP significantly increased the phagocytosis of RAW264.7 macrophage cells. AAP can induce the production of pro-inflammatory cytokines such as TNF-α and IL-6. Chen et al. (2020) isolated three polysaccharide fractions (F1, F2 and F3) from L. edodes. The molecular weights of F1, F2, and F3 are 136 kDa, 14-61 kDa, and 14-
35 kDa, respectively. F1 only had significant effects in increasing thymus index, DTH and proliferation of T splenocytes, while F2 and F3 had broader effects e.g. increasing thymus index, DTH, proliferation of T splenocytes, the content of IgM in serum and splenic NK cytotoxic activity. Smaller molecular weight polysaccharides are likely more easily absorbed and utilized, and can more readily enter cells to promote the proliferation and differentiation of immune cells. This suggests that smaller molecular weight polysaccharides may have a more favorable effect on immunomodulatory activity
limited. Structural characteristics revealed that Auricularia auricula polysaccharide (AAP) was a homogeneous galactan comprising mannose, rhamnose, gluconic acid, glucose, galactose, arabinose, and fucose, and the average molecular weight is approximately 23.51 kDa. At
concentrations of 0.3-0.5 mg/ml, AAP does not produce toxicity in RAW 264.7 cells. After adding AAP, the increase in NO release from the RAW 264.7 cells does not lead to excessive inflammatory responses. The addition of AAP significantly increased the phagocytosis of RAW264.7 macrophage cells. AAP can induce the production of pro-inflammatory cytokines such as TNF-α and IL-6. Chen et al. (2020) isolated three polysaccharide fractions (F1, F2 and F3) from L. edodes. The molecular weights of F1, F2, and F3 are 136 kDa, 14-61 kDa, and 14-
35 kDa, respectively. F1 only had significant effects in increasing thymus index, DTH and proliferation of T splenocytes, while F2 and F3 had broader effects e.g. increasing thymus index, DTH, proliferation of T splenocytes, the content of IgM in serum and splenic NK cytotoxic activity. Smaller molecular weight polysaccharides are likely more easily absorbed and utilized, and can more readily enter cells to promote the proliferation and differentiation of immune cells. This suggests that smaller molecular weight polysaccharides may have a more favorable effect on immunomodulatory activity
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