The Bactericidal Effect of Chlorine-containing Solutions After Plasma Activation
學生姓名:
程郁琇
指導教授:
陳泰源
學期:
113上
摘 要:
Plasma interacts with water to produce plasma-activated water (PAW). During the generation process, the ions generated by plasma interact with water molecules, resulting in complex chemical reactions in the solution, which in turn produces killing various microbial reactive oxygen and nitrogen species (RONS) to extend the shelf life of food. This study studied the bactericidal effect of plasma-activated acidic electrolyzed water (PA-AEW) on Bacillus subtilis suspension and biofilm. The experimental results show that PAW and AEW have poor bactericidal effects on B. subtilis under short-term action, which are 0.98 and 0.58 log CFU/mL respectively, while PA-AEW can cause damage and rupture to the cells of B. subtilis, and its bactericidal effects The significant difference in effect is 2.33 log CFU/mL; bacterial biofilm is often the cause of food contamination, and in the biofilm sterilization experiment under the same action time, PAW (1.46 log CFU/mL), AEW (0.68 log CFU/mL ) and PA-AEW (2.41 log CFU/mL), PA-AEW has the best effect among the three effects, indicating that there is a synergistic effect between reactive chlorine species (RCS) and RONS in sterilization. Plasma26 activated seawater (PASW) presents a promising approach for marine fish preservation. This study found that PASW (PASW-600 = 6.99 log CFU/mL) exhibits superior bactericidal properties against the fish spoilage bacterium Shewanella putrefaciens compared to plasma29 activated water (PAW-600 = 3.02 logCFU/mL) , and increased effectiveness in preserving fish fillets. Findings showed greater nitrite and hydrogen peroxide production in PASW relative to PAW, as well as the conversion of chloride and bromide ions into active species, which collectively enhanced PASW's antimicrobial activity. The synergistic action of RONS and active chlorine and bromine species in PASW promoted the antibacterial.