Exploring the potential of major viral capsid protein vaccine development
學生姓名:
黃智唯
指導教授:
林泓廷
學 期:
113下
摘 要:
In recent years, viruses have caused significant economic losses to the aquaculture industry, making the resolution of emerging viral diseases crucial. Currently, vaccines remain the best method for controlling viral outbreaks. In this study, an experimental recombinant major capsid protein (MCP) vaccine, resembling the damselfish virus, was prepared and tested for its effectiveness in marine ornamental fish. A crude protein compound derived from E. coli BL21 (DE-3) carrying an MCP insert was used to evaluate the efficacy of intraperitoneal injection. Following the SRDV challenge test, the recombinant protein vaccine exhibited only marginal protection based on the recorded relative percent survival (RPS) values from both vaccinated and unvaccinated hermaphroditic populations. Numerous studies have shown that the granular protein of iridovirus of Taiwan (TGIV) is an effective antigen capable of inducing specific immune responses in grouper. However, traditional vaccines based on large proteins face challenges due to unnecessary antigenic load. To address this issue, major linear epitopes within TGIV MCP were screened, and a novel peptide vaccine (P2) was developed using a prokaryotic expression system. Additionally, single-walled carbon nanotubes (SWCNTs) were utilized as a vaccine carrier to enhance immune protection. The results demonstrated a significant increase in the expression levels of immune30 related genes and survival rates. This peptide vaccine effectively induced specific immune responses in vaccinated groups. Functionalized SWCNTs can serve as carriers for peptide vaccines, enhancing immune protection through immersion-based administration.Epitope screening may serve as a potential approach for developing effective vaccines, while SWCNTs could provide a practical method for large-scale vaccination, particularly for disease prevention in juvenile fish.
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