探討使用發酵槽培養大腸桿菌並以自動誘導法生產重組酵素的條件
學生姓名:
陳義洋
指導教授:
方翠筠
學 期:
114上
摘 要:
High fidelity DNA polymerase from Pyrococcus furiosus (Pfupol) is in great demand for biotechnological applications, optimizing Pfupol production is essential to supplying the industry’s expanding demand. D-Allulose 3-epimerase (DAE) is the key enzyme catalyzing D-fructose into D-allulose, a rare sugar in foods, which has lately drawn increasing worldwide attention owing to its possible health advantages and application as a substitute of sucrose. This work focused on the development of an economical, scalable production method of enzyme by using the Escherichia coli BL21 star™ (DE3) as expression host. T7 expression systems is used to express recombinant enzyme in Escherichia coli; optimize the production of the enzyme through an auto-induction strategy in chemically defined media by using lactose as a natural inducer, thereby overcoming various limitations of conventional IPTG induction methods. In 5 L bioreactor auto-induction-based strategy demonstrated its potential for large scale production of enzyme in a cost-effective manner with enhanced reproducibility, which makes it an economically viable and practically useful approach.
碩士班專題討論2025.09.17陳義洋的摘要.pdf