Probiotics Alleviate Reproductive Damage Induced by Polystyrene Microplastics in Male Rats
學生姓名:
曾貴澤
指導教授:
龔瑞林
學期:
110下
摘 要:
Normal regulation of the mammalian hypothalamic-pituitary-gonadal axis is critical for normal reproductive capacity and representation of secondary sexual characteristics. However, a variety of factors can lead to abnormal reproductive system, including eating habits, sleep quality, chronic diseases, environmental hormones, gut microbes, etc. Microplastics refer to plastic debris, particles or thin films with a diameter less than 5 mm, which can be divided into primary and secondary microplastics. Due to their small size and low degradation rate, they are easily ingested and accumulated by various organisms. Therefore, the purpose of this experiment is to explore the damage of polystyrene microplastics (PS-MP) to the reproductive system of male rats, and to investigate the improvement effect of probiotics on this damage. After pretreatment of PS-MP samples, ZETA potential was measured and particle size was observed with Scanning Electron Microscope (SEM). Then, the viability of PS-MP on Raw264.7 and LC-540 cells was determined. Reactive oxygen species (ROS), nitric oxide (NO) production, and testosterone concentrations can be determined after finding the lowest harmful concentration. The results show that the PS-MP has a particle size of about 0.42, a potential of -32.85mV, and a very low PDI value. Cell experiments showed that at the lowest harmful concentration, ROS and NO increased, and the testosterone concentration of LC-540 was significantly lower than that of the control group. The 7- week-old SD rats were divided into control groups, tube-fed 1、5、10 mg/kg PS-MP, mg/kg PS-MP with 108 CFU/day probiotics and only 108 31 CFU/day probiotics. After 6 weeks of tube feeding, sacrifice them and take out blood, liver, testes, epididymis, etc. for biochemical and histological analysis. The results showed that PS-MP reduced sperm count, motility, and increased the percentage of abnormal sperm. In addition, it also significantly damaged the testicular tissue and reduces blood concentration of sex hormones, however, the administration of probiotics can improve reproductive damage. Conclusively, PS-MP can indeed cause reproductive system damage in male rats, which can be improved by probiotics.
專題討論20220427_曾貴澤_摘要.pdf