The effects of Ganoderma lucidum triterpenoids on liver injury and 2 inflammatory responses
摘 要: Oxidative stress and ethanol (EtOH) are the main factor leading to alcoholic liver injury. Triterpenoid of Ganoderma lucidum (G. lucidum) have effects of anti-oxidation and anti-inflammation. In this study, ethanol extract of G. lucidum (GLE) and triterpenes are used to investigate liver protection and anti-inflammatory mechanism of ethanol extract in both in vivo and in vitro. In vivo, the results indicate that activities of aspartate aminotransferase and alanine transaminase serum in mice treated with 30 mg/kg bw GLE tissue are significantly decreased and the damaged liver tissue can be repaired. In addition, Cytochrome P450 2E1 of GLE/EtOH group was significantly decreased compared with EtOH group, while alcohol dehydrogenases 1 was significantly increased, indicating anti-inflammatory effect of GLE. In macrophage Ana-1 cell experiments, G. lucidum and triterpenoids showed protective effects on liver cells and ability of anti-inflammatory. Furthermore, they are not toxic to macrophage Ana-1. Ganodermanontriol inhibits lipopolysaccharide induced inflammatory reaction by inhibiting the interaction Toll-like receptor 4 (TLR4) and myeloid differentiation factor 88 (MyD88), which prevent Nuclear factor kappa B from moving to nucleus. Besides, it prevents mitogen-activated protein kinase pathway. Ganodermanontriol prevents LPS/D-galactosamine-induced liver injury in animal experiments. In conclusion, GLE has the potential of being material, which has effects of liver protection and anti-inflammatory response. Moreover, ganodermanontriol avoids inflammation by inhibiting the interaction between TLR4 and MyD88.