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In vitro and in vivo evaluation fish gelatin and gelatin hydrolysate in opti-mal condition extraction of Hyp-containing peptide concentration

學生姓名: 彭鈺婷
指導教授: 張祐維
學期: 111上
摘  要: In the process of aquatic product processing, a large amount of waste will be generated, and most of these wastes are fish heads, fish bones, fish scales, fish skins, etc. After extraction, gelatin can be obtained, and after hydrolysis of gelatin, it can be found that it has antioxidant and anticancer activity. This study will find the optimal extraction conditions of gelatin, the bioactivity of its hydrolyzate, and further evaluate the concentration of free Hyp, Pro-Hyp and Hyp-Gly in blood after digestion and absorption of gelatin hydrolyzate with different molecular weights. The results showed that the antioxidant activity of gelatin hydrolyzate was 67.75 ± 0.48% and the anticancer activity was 96.93 ± 2.08%. The identified peptide sequence with higher bioactivity was DAGLVGPREGKAGSGPGPYGPEGVFLPMGPR. The concentration
of free Hyp, Pro-Hyp and Hyp-Gly in human blood will increase. The results show that the gelatin hydrolyzate contains active peptides that can be digested and absorbed by the human body, and has the potential to develop functional food raw materials.
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