Detection of viable but non-culturable of Vibrio parahaemolyticus in biofilms formed under seafood processing environments
學生姓名:
陳字雲
指導教授:
蕭心怡
學期:
111上
摘 要:
The aim of this study was to review the current research on the detection of viable but nonculturable (VBNC) state of V. parahaemolyticus in seafood processing environments. The investigation was done by investigating V. parahaemolyticus cells entering the VBNC state at
different incubation temperatures (4oC, 8oC, 37 oC, -18 oC, -45 o C) in Tryptic Soy Broth with salt (TSB), and artificial seawater (ASW). Those state were detected using qPCR, Loop mediated isothermal amplification (qLAMP) wih or without PMA (propidium monoazide),
and plate count (including selective agar and overlayed agar). The results of this review shows that (i) the use of TCBS/TSAS agar shows a higher detection of bacterial counts on agar plates compared to single layer agar, (ii) the use of ASW could damage the membranes of V.parahaemolyticus at 8o C, (iii) bacterial cell viability decreased due to low temperatures and unsuitable growth medium, while exposure time significantly increased the presence of damaged cells in pathogen inactivation, (iv) subzero exposure demonstrated significantly
higher membrane damage in cells than low temperature exposure one, (v) PMA-LAMP could be used as an alternative detection method for pathogen viability after PMA-qPCR. Finally, TCBS/TSAS and PMA-LAMP demonstrated alternative detection methods for V. parahaemolyticus growth. This information could be used as an alternative option by food manufacturers, and epidemiological studies.
different incubation temperatures (4oC, 8oC, 37 oC, -18 oC, -45 o C) in Tryptic Soy Broth with salt (TSB), and artificial seawater (ASW). Those state were detected using qPCR, Loop mediated isothermal amplification (qLAMP) wih or without PMA (propidium monoazide),
and plate count (including selective agar and overlayed agar). The results of this review shows that (i) the use of TCBS/TSAS agar shows a higher detection of bacterial counts on agar plates compared to single layer agar, (ii) the use of ASW could damage the membranes of V.parahaemolyticus at 8o C, (iii) bacterial cell viability decreased due to low temperatures and unsuitable growth medium, while exposure time significantly increased the presence of damaged cells in pathogen inactivation, (iv) subzero exposure demonstrated significantly
higher membrane damage in cells than low temperature exposure one, (v) PMA-LAMP could be used as an alternative detection method for pathogen viability after PMA-qPCR. Finally, TCBS/TSAS and PMA-LAMP demonstrated alternative detection methods for V. parahaemolyticus growth. This information could be used as an alternative option by food manufacturers, and epidemiological studies.
11132055-Seminar20221130-Abstract(Final).pdf