Production of chitooligosaccharides by polyhydroxyalkanoates immobilized recombinant chitosanase
學生姓名:
陳楷雯
指導教授:
方翠筠
學期:
111下
摘 要:
Immobilization of enzymes to solid supports or their self-assembly into insoluble particles enhances their applicability by strongly improving properties such as stability in changing environments, re-usability and applicability in continuous biocatalytic processes. In this study, the csn gene encoded chitosanase CSN was fused to the phaC gene from Ralstonia eutropha H16 by pipe cloning method. Then, the fused gene was co expressed with the phaAB gene to produce polyhydroxyalkanoate (PHA) nanoparticles for the immobilization of CSN. The enzyme was immobilized on the surface of PHA nanoparticles to form PHA-CSN. These nanoparticles possess enzymatic properties
similar to those of free CSN. PHA-CSN has the highest activity at 45℃ and pH 5.5 in the presence of 4 mM Mn2+ . The main hydrolysis products of chitosan catalyzed by PHA CSN are chitobiose and chitotroise.
similar to those of free CSN. PHA-CSN has the highest activity at 45℃ and pH 5.5 in the presence of 4 mM Mn2+ . The main hydrolysis products of chitosan catalyzed by PHA CSN are chitobiose and chitotroise.
陳楷雯_20230510_摘要.pdf