Neuroprotective effect of fermented spent coffee grounds extract using SH-SY5Y human neuronal cell model
學生姓名:
王建薇
指導教授:
蔡國珍
學期:
111下
摘 要:
Parkinson's disease (PD) is a neurodegenerative movement disorder caused by dopamine deficiency, α-synuclein misfolding, mitochondria dysfunction, and oxidative stress. Hydroxydopamine (6-OHDA) is a dopamine neurotoxin that induces the production of reactive oxygen species (ROS), leading to oxidative stress and cell damage or death, and it's often used to induce PD cell patterns. Spent coffee grounds (SCG) contain chlorogenic acid, which protects neuronal cells against oxidative damage caused by H2O2. This study investigated the protection of fermented SCG against oxidative stress caused by H2O2 and 6-OHDA. Seven strains of lactic acid bacteria fermented SCG hydrolysate using xylanase, and FKR2526 and FLC2528 showed the highest growth counts of 9.11 and 9.16 log CFU/mL, respectively. FLC2528 had the highest total phenol and total flavonoid content, with a 46% and 17.38% increase, respectively, compared to unfermented SCG hydrolysate. The fermented SCG freeze-dried powders of FKR2526 and FLC2528 were extracted using microwave-assisted ethanol extraction at different concentrations. The highest concentration of total phenols and total flavonoids was observed in the 20% ethanol extraction. The fermented SCG extracts significantly increased the viability of H2O2- or 6-OHDA-treated SH-SY5Y cells. They also increased intracellular superoxide dismutase (SOD) activity (63.72-129.02%), glutathione peroxidase (GPx) activity (13.10-23.93%), and catalase (CAT) activity (36.57-98.55%). Additionally, they significantly reduced intracellular acetylcholinesterase (AChE) activity (14.21-34.28%). In summary, SCG xylanase enzyme hydrolysate fermented by lactic acid bacteria FKR2526 and FLC2528 could increase total phenol and total flavonoid content and protect against H2O2- and 6-OHDA-induced oxidation by increasing intracellular SOD and CAT activity and inhibiting AChE activity, with the potential for neuroprotective effects and prevention of PD.
C班專討20230510_王建薇.pdf