Purification of Angiotensin I-Converting Enzyme (ACE) Inhibitory Peptides of Molecular Docking Analysis and Antihypertensive Effect of Algae Hydrolysates
學生姓名:
鄭筑軒
指導教授:
陳冠文
學 期:
112上
摘 要:
As about 9.4 million patients die from hypertension worldwide every year. When angiotensin I is exposed to angiotensin I converting enzyme (ACE), resulting in the formation of angiotensin II causes blood pressure to rise. Algae protein has high nutritional value and can
be used as a good source of natural plant protein and bioactive peptides with ACE inhibitory activity. Sargassum maclurei protein hydrolysates (SMPHs) was purified by gel chromatography and LC-ESI-MS/MS. The major peptide sequence RWDISQPY exhibited the
highest ACE inhibitory activity (IC50 = 72.24 µM), it serve as a competitive ACE inhibitor. Finally, in vivo data showed that RWDISQPY administration of significantly reduced SBP and DBP in SHRs. Isochrysis zhanjiangensis microalgae was successfully purified and identify a
novel peptide, FEIHCC (IC50 = 61.38 μM) exhibited significant inhibition activity on ACE. it serve as a mixed competitive ACE inhibitor. The data show that incubation of Peptide Isochrysis Zhanjiangensis (PIZ) inhibited oxidative stress and blunted vascular injury in an Ang II stimulated model of hypertension. In conclusion, the purified peptides isolated from algae protein hydrolysates have potential antihypertensive properties which could potentially be used as functional food ingredients.
be used as a good source of natural plant protein and bioactive peptides with ACE inhibitory activity. Sargassum maclurei protein hydrolysates (SMPHs) was purified by gel chromatography and LC-ESI-MS/MS. The major peptide sequence RWDISQPY exhibited the
highest ACE inhibitory activity (IC50 = 72.24 µM), it serve as a competitive ACE inhibitor. Finally, in vivo data showed that RWDISQPY administration of significantly reduced SBP and DBP in SHRs. Isochrysis zhanjiangensis microalgae was successfully purified and identify a
novel peptide, FEIHCC (IC50 = 61.38 μM) exhibited significant inhibition activity on ACE. it serve as a mixed competitive ACE inhibitor. The data show that incubation of Peptide Isochrysis Zhanjiangensis (PIZ) inhibited oxidative stress and blunted vascular injury in an Ang II stimulated model of hypertension. In conclusion, the purified peptides isolated from algae protein hydrolysates have potential antihypertensive properties which could potentially be used as functional food ingredients.
20231108-專題討論-摘要-鄭筑軒.pdf