Evaluation of the inhibitory effect of Sarcodia suiae polysaccharides on pseudorabies virus infection
學生姓名:
陳妍汎
指導教授:
黃崇雄
學期:
112下
摘 要:
Taiwan, surrounded by the sea, boasts abundant and easily accessible algae resources that are both low-cost and low in toxicity. Certain seaweeds, such as Sarcodia suiae, is rich sulfated polysaccharides with antiviral potential against various enveloped viruses. Therefore, this study aims to investigate the inhibitory effect of S. suiae polysaccharide extract (SSP) on pseudorabies virus (PRV) infection through in vitro. SSP primarily consists of carbohydrates, with a content of 424.7±38.6 mg/g. High-performance liquid chromatography (HPLC) was employed to determine the molecular weight. The analysis revealed that SSP mainly comprises polysaccharide molecules from 90749 to 513314 Da. Fourier-transform infrared spectroscopy (FT-IR) analysis confirmed the presence of variuos functional groups, including sulfate groups. The results of cell viability assessment using the alamar blue reagent indicated no observed toxicity to PK-15 cells (500, 250, 125, 62.5 μg/mL) . Cells were treated with SSP at various growth stages, including PRV (Multiplicity of infection (MOI)=0.1) adsorption, entry, and replication. Subsequently, the presence of SSP was detected using real-time quantitative polymerase chain reaction (qPCR). The experimental findings validated that pre- and post-virus infection addition of SSP exhibited the most potent virus-inhibiting effect. These results suggest that SSP possesses antiviral properties against PRV and may serve as a promising adjuvant treatment option in the future.
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