Using Atopic Dermatitis Mice Model to Study the Improving Effects and Concerted Mechanism of Undaria pinnatifida Water Extracts and Sacha Inchi Oil
學生姓名:
黃璟瑀
指導教授:
吳彰哲
學期:
111下
摘 要:
Atopic dermatitis (AD) is an allergic skin disease accompanied by chronic inflammation that is characterized by severe itching, redness, dryness, and eczematous skin lesions. It is known to be caused by immune dysregulation resulting from the complex interaction of environmental and genetic factors. Undaria pinnatifida is an edible brown seaweed which has bioactivities such as antioxidant, anti-inflammatory, antitumor, antiviral, and anti-obesity properties. Sacha inchi (Plukenetia volubilis) oil (SIO) has high contents of omega-3 fatty acids that can prevent multiple diseases; including arthritis, cancer, diabetes, and inflammatory skin diseases. Mice were divided into seven groups: (1) Control (only soybean oil), (2) DNCB-ddH2O (DNCB + ddH2O), (3) DNCB-soybean oil (DNCB + soybean oil), (4) Dex. (DNCB + Dexamethasone), (5) UPw (DNCB + Undaria pinnatifida water extracts), (6) SIO (DNCB + Sacha inchi oil) and (7) Mix (DNCB + Undaria pinnatifida water extracts + Sacha inchi oil) .The results showed that the antioxidant DPPH scavenging activity, hydrogen peroxide scavenging activity and ferrous ion chelating activity of Undaria pinnatifida water extracts (UPw) were 77.33 ± 0.74 %, 70.79 ± 2.72 % and 86.80 ± 0.94 %, while the antioxidant DPPH scavenging activity, and ferrous ion chelating activity of SIO methanol extracts were 60.33 ± 2.60 % and 66.87 ± 8.64 %. When used in vitro 1 mg/mL of UPw and 3.125 mg/g of SIO with P815 mouse mast cells had anti-degranulation effect, but not significant cytotoxicity. In vivo, UPw, SIO, and their combination showed they could reduce DNCB-induced IgE expression, skin damage, subiliac lymph node swelling, and spleen swelling while having no synergistic effects on alleviating DNCB-induced symptoms in BALB/c mice. It suggests that UPw and SIO could serve as therapeutic agents for inflammatory illnesses of AD.
專題討論_20230329_摘要-黃璟瑀.pdf