Effects of Different Extraction Methods on the Contents of Type II Collagen and Chondroitin Sulfate in Chicken Sternal Cartilage and Sturgeon Notochord
學生姓名:
李佳昕
指導教授:
陳冠文
學 期:
114上
摘 要:
Chondroitin sulfate (CS) and undenatured type II collagen (Col II) are the two major biological macromolecules found in cartilage-related tissues. This study investigated the extraction techniques and structural characteristics of Col II and CS obtained from chicken sternal cartilage (CSC) and sturgeon (Acipenser gueldenstaedti) notochord by-products. The results showed that both enzymatic hydrolysis and alkaline treatment effectively yielded CS and Col II. The enzymatically extracted Col II consisted mainly of α₁ (130 kDa) and β (270 kDa) chains, while Fourier-transform infrared spectroscopy (FTIR) confirmed that its triple-helical structure remained intact after processing. The average molecular weight of CS ranged between 38 and 80 kDa, and the predominant form was chondroitin-4-sulfate (CS-A). Differential scanning calorimetry (DSC) and scanning electron microscopy (SEM) analyses revealed that Col II exhibited a fibrillar structure, whereas CS appeared as rough, granular aggregates; together, they formed stable hybrid fibrils. These findings demonstrate that CSC and sturgeon notochord are promising sources of Col II and CS, providing a scientific basis for the valorization of animal by-products.
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