Exploring the Potential and Mechanisms of Natural Proteins and Synthetic Drugs Against SARS-CoV-2
學生姓名:
蔡幸倫
指導教授:
吳彰哲
學 期:
114上
摘 要:
SARS-CoV-2 has caused the global COVID-19 pandemic. Although remdesivir was the first antiviral approved for clinical use, its therapeutic efficacy remains controversial, prompting further investigation of its primary active antiviral component. In addition, SARS-CoV-2 RNA has been detected in the breast milk of infected mothers, but infectious virus particles have never been isolated, suggesting that there may be antiviral components in breast milk. These two studies respectively examined the antiviral potential of GS-441524-the parent nucleoside of remdesivir and the naturally occurring milk protein bovine lactoferrin ( bLf ) derived from milk against SARS-CoV-2, as well as their possible mechanisms.
In cell experiments and animal models, GS-441524 can effectively inhibit SARS23 CoV-2 activity and reduce tissue damage, while its production cost and efficacy are also lower compared to remdesivir. This study also developed the first SARS-CoV-2 animal model in AAV-hACE2 mice using GS-441524. The second study shows that bLf and milk fat globule membrane ( MFGM ) are identified as the main antiviral components in breast milk. Among them, bLf can block the interaction between the spike protein and ACE2, inhibit viral RNA replication, and has been shown to directly inhibit the RNA polymerase ( RdRp ) of SARS-CoV-2.
In cell experiments and animal models, GS-441524 can effectively inhibit SARS23 CoV-2 activity and reduce tissue damage, while its production cost and efficacy are also lower compared to remdesivir. This study also developed the first SARS-CoV-2 animal model in AAV-hACE2 mice using GS-441524. The second study shows that bLf and milk fat globule membrane ( MFGM ) are identified as the main antiviral components in breast milk. Among them, bLf can block the interaction between the spike protein and ACE2, inhibit viral RNA replication, and has been shown to directly inhibit the RNA polymerase ( RdRp ) of SARS-CoV-2.
蔡幸倫.pdf