Effects of Fermented Extracts on Anti-Aging and Antioxidant Activities in Caenorhabditis elegans
學生姓名:
蘇家靖
指導教授:
陳詠宗
學期:
115上
摘 要:
Microbial fermentation can modify the chemical composition and biological activity of food-derived materials, potentially enhancing their effects on healthy aging. However, the extent to which different fermented food-derived extracts influence longevity and the molecular mechanisms underlying these effects remain incompletely understood. The anti-aging and antioxidant effects of fermented soybean and green tea-derived extracts were therefore evaluated using Caenorhabditis elegans as an in vivo model. Tempeh extract significantly extended the lifespan of wild-type N2 worms, with the greatest increase observed at 100 μg/mL, while also improving age-related locomotor function and reducing intracellular reactive oxygen species. Genetic analysis further demonstrated that its longevity-promoting effect required the PMK-1–SKN-1 signaling axis of the MAPK pathway. Similarly, liquid-state fermentation of green tea extract with Aspergillus niger RAF106 markedly enhanced its anti-aging activity, with the 4 day fermented extract producing the strongest effects on lifespan, motility, stress resistance, mitochondrial function, and antioxidant status. This treatment reduced ROS and MDA levels while increasing SOD, CAT, and GSH, and its longevity-promoting activity involved IIS, MAPK, JNK, ERK, and p53-related pathways together with DAF-16 and HSF-1. Untargeted metabolomic profiling further revealed substantial fermentation- induced changes in the chemical composition of green tea extract, and several metabolites enriched after fermentation, including proanthocyanidin A2, aromadendrin, and dalbergioidin, were independently confirmed to extend lifespan in C. elegans. The anti-aging potential of extracts derived from different fermented natural substrates is reflected in their ability to extend lifespan, improve healthspan-related phenotypes, reduce oxidative stress, and modulate longevity-associated signaling pathways in C. elegans.
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