Influence of Fish Species, Farming Environment, and Culture Conditions on Fish Fatty Acid Composition
學生姓名:
李宜蓁
指導教授:
方銘志
學期:
115上
摘 要:
Quality control and origin authentication of aquaculture and wild fish products increasingly rely on fatty acid profiling because muscle fatty acid composition reflects the combined influences of genetics, environmental conditions, and diet. Previous studies have shown that fatty acid signatures can effectively distinguish production systems, feeding practices, and geographic origins, providing a reliable basis for
traceability and authentication.
In rainbow trout (Oncorhynchus mykiss), comparisons between Russian aquaculture farms and wild populations demonstrated that both geographic conditions and genetic strains influence lipid deposition. Fish with later spawning seasons exhibited higher EPA and DHA accumulation, while clear differences were observed between cultured and wild trout. Farmed trout contained markedly higher levels of linoleic acid (18:2n-6) due to vegetable oil supplementation, whereas wild populations maintained much lower concentrations, making fatty acid profiles effective indicators for distinguishing farming history and origin.The importance of diet was further demonstrated in largemouth bass (Micropterus salmoides). Analysis of commercial pond samples revealed a strong relationship between feed type and fillet fatty acid
composition. Fish fed frozen trash fish accumulated higher levels of marine-derived ω 3 fatty acids, particularly EPA and DHA, whereas those fed formulated diets showed increased levels of plant-derived fatty acids such as C18:1n-9 and C18:2n-6. These differences produced distinct fatty acid patterns, confirming feed composition as a major factor influencing lipid profiles.The application of fatty acid profiling for origin verification was further validated in cultured red seabream (Pagrus major) from Korea and Japan. Differences in feeding practices resulted in distinct fatty acid compositions, while amino acids and minerals showed limited discriminatory ability. Among all variables examined, linoleic acid (C18:2n-6) was identified as the most effective biomarker, with a threshold of 6.45% successfully separating Korean and Japanese products and achieving 100% classification accuracy in blind tests. Collectively, these studies demonstrate that fatty acid composition is strongly shaped by genetics, environment, and feed formulation. Specific fatty acids, particularly C18:2n-6, EPA, and DHA, consistently differentiate culture systems, feeding regimes, and geographic origins, highlighting the value of fatty acid profiling as a practical tool for traceability, origin authentication, and quality control in aquaculture products.
traceability and authentication.
In rainbow trout (Oncorhynchus mykiss), comparisons between Russian aquaculture farms and wild populations demonstrated that both geographic conditions and genetic strains influence lipid deposition. Fish with later spawning seasons exhibited higher EPA and DHA accumulation, while clear differences were observed between cultured and wild trout. Farmed trout contained markedly higher levels of linoleic acid (18:2n-6) due to vegetable oil supplementation, whereas wild populations maintained much lower concentrations, making fatty acid profiles effective indicators for distinguishing farming history and origin.The importance of diet was further demonstrated in largemouth bass (Micropterus salmoides). Analysis of commercial pond samples revealed a strong relationship between feed type and fillet fatty acid
composition. Fish fed frozen trash fish accumulated higher levels of marine-derived ω 3 fatty acids, particularly EPA and DHA, whereas those fed formulated diets showed increased levels of plant-derived fatty acids such as C18:1n-9 and C18:2n-6. These differences produced distinct fatty acid patterns, confirming feed composition as a major factor influencing lipid profiles.The application of fatty acid profiling for origin verification was further validated in cultured red seabream (Pagrus major) from Korea and Japan. Differences in feeding practices resulted in distinct fatty acid compositions, while amino acids and minerals showed limited discriminatory ability. Among all variables examined, linoleic acid (C18:2n-6) was identified as the most effective biomarker, with a threshold of 6.45% successfully separating Korean and Japanese products and achieving 100% classification accuracy in blind tests. Collectively, these studies demonstrate that fatty acid composition is strongly shaped by genetics, environment, and feed formulation. Specific fatty acids, particularly C18:2n-6, EPA, and DHA, consistently differentiate culture systems, feeding regimes, and geographic origins, highlighting the value of fatty acid profiling as a practical tool for traceability, origin authentication, and quality control in aquaculture products.
李宜蓁.pdf