Osteogenic effect of 3D printed porous hydroxyapatite scaffold by microwave sintering with embedded rhBMP-2
學生姓名:
李孟臻
指導教授:
黃意真
學期:
105下
摘 要:
Recently, 3D printing as effective technology has been highlighted in the biomedical field. Previously, aporous hydroxyapatite (HA) scaffold with the biocompatibility and osteoconductivity has been developed by this method and then sintering in a microwave furnace. The purpose of this study was to investigate the effects of microwave sintering on the physical and biological properties of porous HA bone scaffolds, and improveit by the introduction of recombinant human bone morphogenetic protein-2 (rhBMP-2). After rapid sintering, scaffolds with controlled structure, high densification and fine grains were obtained. A significant increase in mechanical strength was observed relative to conventional sintering. The scaffolds microwave sintered at 1200℃ for 30 min exhibited the highest average compressive strength (45.57 MPa). The results indicated that the microwave-sintered scaffolds possessed higher solubility than conventionally sintered scaffolds. Furthermore, an in vitro MC3T3-E1 cell culturing study showed significant cell adhesion, distribution, and proliferation in the microwave-sintered scaffolds. However, its osteoinductivity is limited. This scaffold was developed by coating rhBMP-2-delivery microspheres with collagen. Scanning Electron Microscopy (SEM) results indicated the surface of scaffolds were more fit for the adhesion of hMSCs to coat collagen/rhBMP-2 microspheres. Biphasic release of rhBMP-2 could continue for more than 21 days, and keep its osteoinductivity to induce osteogenic differentiation of hMSCs in vitro. The experiments in vivo showed that the scaffold had a good bone regeneration capacity. These results confirm that microwave sintering has a positive effect on the properties of porous hydroxyapatite scaffolds, and microspheres system can simultaneously achieve localized long-term controlled release of rhBMP-2 and bone regeneration, which provides a promising route for improving the treatment of bone defects for bone tissue engineering applications.
李孟臻專討摘要20170322.pdf