Enhancing the Growth Rate and Astaxanthin Yield of Haematococcus pluvialis by High Light Intensity, High Concentration of Carbon Dioxide, Nuclear Irradiation or Biofilm Cultivation
學生姓名:
鄭景龠
指導教授:
潘崇良
學 號:
10532007
學期:
105下
摘 要:
State-of-the-art commercial cultivation of Haematococcus pluvialis for astaxanthin production is exclusively based on two-phase processes, separating a vegetative “green” phase for cell multiplication from an astaxanthin producing “red” phase consisting of resting stages. By means of a Twin-Layer biofilm photobioreactor,H. pluvialis was grown immobilized under illuminations up to 1,015 μmol photons m−2 s−1 and supplementary CO2 in the range of 1% to 10%, reaching a maximal dry biomass productivity of 19.4 g m−2 day−1 and a final standing crop of 213 g m−2 growth area after 16 days of cultivation. During the cultivation period, astaxanthin productivity increased with increasing light intensity to values of 0.39 g m−2 day−1 and a final amount of 3.4 g m−2, representing an astaxanthin content of about 2.5% in the dry biomass. H. pluvialis FACHB-872 was mutated with 60Co-γ irradiation to promote growth rate and increase astaxanthin yield under high concentration of CO2 stress. The average specific growth rate of H. pluvialis mutated with 4,000 Gy γ-ray irradiation was increased by 15% compared with the original strain with air aeration. The mutant grew best with 6% CO2 (the maximum specific growth rate was 0.60/d) when it was cultured with high concentrations of CO2 (2-10%). The peak biomass productivity (0.16 g/L/d) of the mutant cultured with 6% CO2 was 82% higher than that of the mutant with air. The astaxanthin yield of the mutant induced with 6% CO2 and high light (108 μmol photons m−2 s−1) increased to 46.0 mg/L, which were 2.4 times higher than those of the wild- type strain.
2017-0220-鄭景龠-摘要-1052專題討論書面報告 .pdf