The application of atmospheric cold plasma treatment against bacterial biofilms
學生姓名:
陳字雲
指導教授:
蕭心怡
學期:
110下
摘 要:
The aim of this study was revealed the efficacy of atmospheric cold plasma (ACP) treatment on controlling or inactivating biofilm formation in food models during the treatment (exposure time, post treatment storage time /PTST, voltage, flow rate), and stress conditions
(growth environment, storage temperature, biofilm age, biofilm thickness). The investigation was done by inoculating either mono or mixed biofilm species (E. coli, S. enterica, L. monocytogenes, P. fluorescens) on fresh lettuce, and E. coli and L. innocua on biphasic (xanthan gum and whey protein) food models. Confocal laser scanning microscopy (CLSM), XTT assays, flow cytometry, and plate counts were used to reveal the efficacy and differences in the quantitative of biofilm population on the samples. The results of this study show that (1) The use of high voltage plasma treatment with PTST and various environmental stress exhibited greater reduction against E. coli mono-culture biofilms. Meanwhile, mixed species of L. monocytogenes and P. fluorescens did take longer exposure times than mono culture to inactivate the biofilm. (2) The use of ions flow (NO2, NO, O3) plasma treatment demonstrated inactivation effect against L. innocua between mono and mixed culture biofilm, but did not show significant reduction effect against E. coli, indicating that biofilms formed by E. coli has high resistance to the disinfection or even did not get stress of the biofilms. In conclusion, the different applied system of ACP and types of food28 borne bacteria can influence the inactivation effect of biofilms.
(growth environment, storage temperature, biofilm age, biofilm thickness). The investigation was done by inoculating either mono or mixed biofilm species (E. coli, S. enterica, L. monocytogenes, P. fluorescens) on fresh lettuce, and E. coli and L. innocua on biphasic (xanthan gum and whey protein) food models. Confocal laser scanning microscopy (CLSM), XTT assays, flow cytometry, and plate counts were used to reveal the efficacy and differences in the quantitative of biofilm population on the samples. The results of this study show that (1) The use of high voltage plasma treatment with PTST and various environmental stress exhibited greater reduction against E. coli mono-culture biofilms. Meanwhile, mixed species of L. monocytogenes and P. fluorescens did take longer exposure times than mono culture to inactivate the biofilm. (2) The use of ions flow (NO2, NO, O3) plasma treatment demonstrated inactivation effect against L. innocua between mono and mixed culture biofilm, but did not show significant reduction effect against E. coli, indicating that biofilms formed by E. coli has high resistance to the disinfection or even did not get stress of the biofilms. In conclusion, the different applied system of ACP and types of food28 borne bacteria can influence the inactivation effect of biofilms.
專題討論20220309-陳字雲（摘要）.pdf