Screening and Mechanistic Evaluation of Antioxidant Lactic Acid Bacteria Using Caenorhabditis elegans with Validation in 3 Mammalian Infection
學生姓名:
李素惠
指導教授:
陳詠宗
學期:
114下
摘 要:
Lactic acid bacteria (LAB) have attracted increasing attention as natural antioxidants. Caenorhabditis elegans has been widely used as an in vivo model for evaluating antioxidant activity due to its short lifespan and well-characterized genetic background. This report summarizes two studies investigating the antioxidant potential of LAB and their protective effects against oxidative stress. In the first study, a mutant strain Lactiplantibacillus plantarum A72 obtained through atmospheric and room20 temperature plasma (ARTP) mutagenesis significantly extended the lifespan of C. elegans and enhanced stress resistance. A72 reduced reactive oxygen species (ROS) and malondialdehyde (MDA) levels while increasing antioxidant enzyme activities (SOD and CAT), and regulated oxidative stress-related genes, indicating improved antioxidant defense in C. elegans. In the second study, multiple LAB strains were evaluated for their ability to protect C. elegans from H2O2- and juglone-induced oxidative stress. Several strains significantly improved nematode survival, enhanced antioxidant enzyme activities, and upregulated oxidative stress-related genes. These effects were further validated in a Campylobacter jejuni-infected mouse model, where effective LAB strains maintained antioxidant capacity and glutathione levels.
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