Self-assembly mechanism of undenatured type II collagen nanofibrils and Ca²⁺ induced crosslinking in sodium alginate-coated systems for functional delivery applications.
學生姓名:
李佳昕
指導教授:
陳冠文
學期:
114下
摘 要:
This study investigated the self-assembly behavior of undenatured type II collagen (UC-II) and its subsequent functional applications. First, UC-II with an intact triple-helical structure was extracted from chicken sternal cartilage via enzymatic methods, and its self19 assembly behavior and structural regulation mechanisms under different concentrations, pH, and temperature conditions were analyzed using atomic force microscopy. The results showed that UC-II formed dense and uniform nanofibril structures under conditions close to its isoelectric point (pH 5.0), and its self-assembly behavior was influenced by concentration and temperature. Structural analysis indicated that the self-assembly was primarily driven by hydrophobic interactions, accompanied by hydrogen bonding and electrostatic interactions. On this basis, sodium alginate (SA) was further introduced, and coating treatments were performed under different Ca²⁺ concentrations (1–9 mM) to evaluate their effects on nanofibril structure and properties. The results showed that an appropriate Ca²⁺ concentration (≤5 mM) could maintain the triple-helical structure of UC-II while enhancing its thermal stability to 65.02°C and significantly improving the structural retention during gastrointestinal digestion. In addition, this system could be further applied for curcumin (Cur) loading, enhancing its water solubility and dispersion stability, and improving its antioxidant activity and release behavior. Overall, this study combined UC-II self-assembly and coating strategies to develop a nanostructured system with structural stability and functional potential.
李佳昕.pdf